Welcome to DMBR
Department of Molecular Biology and Radiobiology!

DMBR - Department of Molecular Biology and Radiobiology focuses primarily on studying the molecular basis of interactions between plants and their environment. We investigate interactions with abiotic and biotic factors using a wide range of "omics" approaches. We have long achieved national and international excellence in these areas, as demonstrated by both our research projects and scientific publications.

Our department has two main research directions:

• Interactions of temperature, light, and plant hormone signaling pathways
  Prof. Brzobohatý, Assoc. Prof. Novák
• Molecular basis of interactions between plants and microorganisms
  Assoc. Prof. Černý

All research directions are closely interconnected and are based on similar methodological foundations, dominated by mass spectrometry techniques followed by bioinformatics analysis. We use mass spectrometry to monitor protein levels, their modifications and interactions, metabolome profiling, lipid analysis, and targeted monitoring of secondary metabolites.

Metabolomics

Thermo Q Exactive GC Orbitrap GC-MS/MS, Thermo, supplemented with equipment for volatile compound analysis

Proteomics and Lipidomics

Equipment for LC-MS omics analyses: Orbitrap Fusion Lumos and Evosep ENO, Thermo Dionex Ultimate 3000 RSLC nano, UHR maXis impact q-TOF mass spectrometer, TSQ Quantiva triple quadrupole mass spectrometer

Cultivation and Measurement

Cultivation chambers supplemented with diode sources of monochromatic light (R, FR, B). Greenhouses with a wide range of light regime regulation. FlourCam Handy FC 1000-H fluorescence camera for measuring photosynthetic parameters.

Transcriptomics

Robotics and complete equipment for transcriptomic analysis

Bioinformatics

Processing complex data from omics analyses and phenotyping