Science

     
   

2025-2026  8J25DE014 s názvem Malé biomolekuly obsahující síru a jejich role v regulaci růstu – nová třída fytohormonů?   

 

 

Prof. Dr. Stanislav Kopřiva

Institute for Plant Sciences, Cologne Biocenter, University of Cologne 

 The bilateral project will have the following objectives that coincide with the planned mobilities: 1) To test whether the growth-promoting effect found in response to sulfur-containing molecules is dependent on plant sulfur nutrient status. The impact of selected biomolecules on plants grown in sulfur-poor and sulfur-rich media will be explored. In parallel to A. thaliana Col-0, we will assess available reporter lines and mutants: (i) hormonal signaling mutants and reporter lines from MENDELU and (ii) sulfur metabolism and uptake mutants from UC. 2) To identify novel regulatory pathways involved in plant responses to sulfur-containing molecules at different concentrations. This objective will utilize proteome analyses, hormonome, metabolome, and lipidome profiling (MENDELU), and ionomics and secondary metabolites analyses (UC). Selected samples will be used for transcriptome profiling, and the corresponding data analysis will be jointly performed by both partners. 3) Priming with sulfur-containing molecules promotes stress resilience. Here we will use the resources of biotic interactors available at UC, plant growth-promoting and pathogenic bacteria and fungi, and quantify the effects of the chemicals on the interaction of plants with these microorganisms. In parallel the priming effects against a range of abiotic stresses – NaCl, osmotic stress, heat stress, and combinations – will be tested by measuring plant performance, levels of stress-related metabolites, and expression of stress marker genes. 4) Validation, summarization of the results, and joint project application. After validating and summarizing the results, we will disseminate our findings through scientific publications and develop a joint project proposal to further our collaboration.
 
 

2023-2025 8X23011 - Identifikace obranných mechanismů proti škodlivým a agresivním patogenům ekonomicky významných dřevin

https://starfos.tacr.cz/projekty/8X23011?query=7r6yaac75cba

 

Prof. Dr. Jaroslav Ďurkovič

Technická univerzita vo Zvolene, SK 

Dr Ivan Milenković

University of Belgrade – Faculty of Forestry 

 

The main objective of this project is to extend the already established collaborations, exchange knowledge via internships, educate talented young scientists, and prepare the foundation of a consortium that could compete for European research projects. The overall research objective is to determine the response of trees at the histological and molecular level following the single and combined attacks of harmful organisms to the respective hosts in the field and mesocosm experiments. Our ambition is to find the biochemical and anatomical mechanisms of tree defense against harmful and aggressive pathogens causing significant economic losses in forests, to reveal new biochemical and phytopathological knowledge, as well as knowledge on the interactions between plants, pathogens, and pests.

 
 
 
   
  
 

2023-2025 GAČR 23-07376S - Modulace tepelné stresové reakce signální dráhou cytokininu u Arabidopsis

https://starfos.tacr.cz/projekty/GA23-07376S?query=xijaaac76bha
Heat stress response (HSR) evolved to protect plants from negative impacts of heat stress (HS) on plants’ fitness and reproduction. Understanding mechanistic bases for HSR is of high importance because of global warming. Hormone cytokinin (CK) is known to affect plant sensitivity to heat stress. However, the specific role of CK signaling components in HSR is still elusive. To get an insight into the subject, effects of modulation of CK levels and signaling on HSR will be investigated in Arabidopsis using a suit of omics approaches. The effects will be analyzed at a whole seedling level first. To address roles of phyllotaxy and ontogenesis, molecular processes underlying HSR will be analyzed in leaf 3 and leaf 6 at the beginning and end of cell expansion phase. Molecular networks governing enhancement of HSR by CK will be revealed. Relative physiological significance of key nodes of the networks will be assessed by genetic and pharmacological approaches. Knowledge gained may form a base for increasing heat stress tolerance in crops within follow-up projects.
 
   
  
 

 2023-2024 8J23DE004 - Role rostlinného hormonu cytokininu v tvorbě sekundárních metabolitů a formování komunikace mezi rostlinou a mikroorganismy.

     https://starfos.tacr.cz/projekty/8J23DE004?query=ukoaaadbetya

Prof. Dr. Stanislav Kopřiva

Institute for Plant Sciences, Cologne Biocenter, University of Cologne 

1) Impact of cytokinin level modulation on the interaction of A. thaliana with growth-promoting bacteria. We will use transgenic A. thaliana lines and pharmacological approaches to modulate endogenous cytokinin levels, and we will monitor the effect of a change in cytokinin homeostasis on the induction of two-way plant-bacteria interactions (e.g., Pseudomonas fluorescens or synthetic communities). Changes will be observed at the level of physiological parameters, production of secondary metabolites, and plant lipidome and proteome, including post-translational modifications of proteins (e.g., phosphorylation and modifications of histones). 2) Impact of cytokinin level modulation on the interaction of A. thaliana with pathogenic bacteria. We will build on the first set of experiments and extend the two-way interaction analyses to pathogenic bacteria (e.g., Burkholderia glumae). We will compare the results of physiological and molecular analyses from both studies and evaluate the role of cytokinins. 3) Genetic analysis of the role of the cytokinin signalling pathway in biotic interactions. Based on the obtained results, we will employ selected mutant plants covering mutations in cytokinin receptors and all steps of the signalling cascade and identify genes that are key for plant communication with microorganisms and the creation of interactions. Using molecular analyses, we will try to reveal the effect of mutations on the observed interactions. 4) Functional validation of identified proteins. We will verify the function of selected candidate proteins (obtained under the first three aims) by analysing the available mutants in the corresponding genes, and we will also monitor their orthologs in crops. Their role will be verified based on the ability of the corresponding mutant plants to establish a beneficial relationship or resist infectious pressure. The interaction will be monitored using physiological analysis or by monitoring molecular biotic stress markers.

 

  
 
   
  
     
 

 2023-2026 Plant pest prevention through technology-guided monitoring and site-specific control

http://www.purpest.eu/
 
Pest invasions are projected to increase as the climate changes. This poses a major threat to food systems. Preventing outbreaks and early detection are a top priority in Europe. In this context, the EU-funded PURPEST project will develop and demonstrate an innovative sensor platform that can rapidly detect five different pests during import and in the field to stop their establishment and reduce pesticide inputs by at least 50 %. The sensor is based on detection of pest-specific volatile organic compounds (VOCs) emitted by pests and/or host plants invaded by pests. The project will determine the VOC signature of plants attacked by Phytophthora ramorum and other Phytophthora species, the fall armyworm, the cotton bollworm, the brown marmorated stink bug and the pine wood nematode under different abiotic stress conditions.

2022-2024 GAČR 22-17092S - Redoxní signalizace na chromatin během stresových reakcí v rostlinách

https://starfos.tacr.cz/projekty/GA22-17092S?query=qrhaaadbmxpa

Adverse environmental conditions trigger extensive transcriptional reprogramming that is partially regulated at the chromatin level which adds critical context for the activity of transcription factors. Chromatin features such as post-translational histone modifications are altered by histone-modifying enzymes in response to external stimuli. In Arabidopsis, the evolutionary conserved SAGA complex, a multi-functional transcriptional co-activator, is involved in histone acetylation and regulates numerous stress-response and developmental programmes. Intriguingly, despite its profound impact on gene expression, the molecular mechanisms regulating the acetylation activity of the SAGA complex are still unknown. We will investigate how redox regulation impacts the genome-wide distribution of histone acetylation marks deposited by the SAGA complex, its protein composition, and targeted genes. Results from the project will reveal central mechanisms for regulation of gene expression under stress conditions and pave the way towards epigenetic engineering of climate-resilient crops.

  

 

  

 

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 2023-2026 CROP PRIME - Stabilising crop yield under unfavourable conditions by molecular priminghttps://www.cropprime.eu/Today’s agricultural industry is required to deliver ever-increasing food and feed amounts under the pressures of the climate crisis. Moreover, consumers and regulators increasingly demand environmentally friendly solutions to combat abiotic and biotic stress associated with yield losses. Biostimulants represent an alternative to synthetic agrochemicals and can sustainably improve crop productivity. A science-based understanding of their beneficial effects as well as avenues for fine-tuning their bioactivities are needed. The EU-funded CropPrime project aims to elucidate the molecular mechanisms underlying stress priming and deliver optimised biostimulants to counteract stress-induced yield losses. To achieve this task, leading plant scientists in abiotic and biotic stress signalling from Europe, Africa and South America will join forces with an industrial partner specialising in biostimulants production and marketing.
  • 2018-2023 

Centrum experimentální biologie rostlin

 

Náplní projektu je objasnit mechanismy, jimiž rostliny reagují na vlivy vnějšího prostředí, zahrnující jak abiotické, tak biotické stresy a jejich kombinace. Šíře přístupů (od buněčné úrovně až po vývoj plodů) a metodik (transkriptomika, proteomika, hormonomika, těkavé látky) umožní získat komplexní obraz dynamické odezvy rostlin na vnější prostředí. Projekt je řešen ve spolupráci s Ústavem experimentální biologie rostlin AV ČR

Název projektu: Centrum experimentální biologie rostlin
Číslo projektu:  CZ.02.1.01/0.0/0.0/16_019/0000738
Termín řešení projektu: 1. 7. 2018 – 30. 6. 2023

  • 2016-2022 

Výzkumné centrum pro studium patogenu z rodu Phytophthora

 Cílem projektu je vybudovat internacionalizovaný excelentní výzkumný tým zaměřený na výzkum patogenů rodu Phytophthora (Chromalveolata, Heterocontophyta, Oomycetes) na dřevinách.  Choroby z rodu Phytophthora způsobují celosvětově více než 66 % chorob kořenového vlášení a více než 90 % chorob kořenového krčku u dřevin. Internacionalizovaný výzkumný tým bude tvořit absolutní světovou špičku výzkumu v této problematice za  využití infrastruktury výzkumného prostředí Brna ve  spolupráci se špičkovými  pracovišti v zahraničí. Řešením projektu bude prohloubeno poznání globální diverzity rodu, jeho evoluční historie, mechanismů mezidruhového křížení a faktorů adaptability stejně jako molekulárních mechanismů, které ovlivňují odolnost a náchylnost dřevin vůči poškození. Získané unikátní poznatky budou následně použity pro řešení ochrany a obrany a zamezení šíření těchto patogenů v rámci přírodních ekosystémů. Výstupy budou poskytnuty subjektům v aplikační sféře zabývajících se pěstováním a distribucí dřevin. Projekt je řešen ve spolupráci s Ústavem ochrany lesů a myslivosti LDF.

Název projektu: Výzkumné centrum pro studium patogenů z rodu Phytophthora
Číslo projektu: CZ.02.1.01/0.0/0.0/15_003/0000453
Termín řešení projektu: 1. 12. 2016 – 31. 10. 2022
Stránky projektu: CZ.02.1.01/0.0/0.0/15_003/0000453 - Výzkumné centrum pro studium patogenu z rodu Phytophthora

  • 2019-2023 

Identifikace metabolitů korelujících s kvantitativní rezistencí k Phytophthora infestans

Cílem řešení projektu je nalezení skupiny metabolitů, které budou fungovat jako biomarkery selekce šlechtitelských materiálů rodu Solanum s horizontální (trvalou) rezistencí nejzávažnějšímu patogenu bramboru Phytophthora infestans. Projekt je řešen ve spolupráci s Výzkumným ústavem bramborářským a VESA Velhartice.

Název projektu: Identifikace metabolitů korelujících s kvantitativní rezistencí k Phytophthora infestans
Číslo projektu: QK1910045
Termín řešení projektu: 1. 1. 2019 – 31. 12. 2023

  • 2/2016 - 12/2020 

CEITEC 2020

Vývojová a produkční biologie - omické přístupy - Břetislav Brzobohatý

 

Jsme součástí centra excelence CEITEC. Více informací najdete zde. 

Participujeme rovněž v CEITEC PhD programu.

 

 

  • 2021-2023 IGRÁČEK - Beating the heat: A system biology approach towards heat-stress resilient crops
  • 2020-2022 GAČR 20-26232S - Interakce mezi chladem a světlem v chladové aklimatizaci Arabidopsis – ontogenní a fylotaktický pohled
  • 2021 IGA - Biologická kontrola jako nástroj pro zmírnění abiotického stresu rostlin
  • 2021 IGA - Vysokorozlišovací omická analýza pro identifikaci lokálních změn v rostlinném pletivu vystaveném účinkům patogenního organismu
  • 2020-2022 8J20DE007 - Role HSP proteinů v infekci Plasmodiophora brassicae.
  • 2018-2019 7AMB18DE015 - Komplexní analýza onemocnění nádorovitosti kořenů a jeho možné biologické kontroly endofytickou houbou.

Prof. Dr. Jutta Ludwig-Müller
Institute for Botany, Technische Universität Dresden (Germany)

 

  • 2017-2019 GAČR 17-04607S - Interakce mezi světlem a cytokininy v kontrastních ekotypech Arabidopsis
RNDr. Radomíra Vaňková, CSc.
Ústav experimentální botaniky AV ČR, v.v.i.
  • 2017-2018 7AMB17AT027 - Cílená degradace proteinů v odpovědi na rostlinný hormon cytokinin. 

Ao. Univ.‐Prof. Dr. Andreas Bachmair
Max F. Perutz Laboratories, University of Vienna (Austria).

  • TÜBITAK/14/012 - Total Leaf and Nuclear Proteome Response to Drought Stress in Tomato Plants with up-regulated Cytokinin Synthesis

Prof. Anne Frary
The Doganlar/Frary Laboratory for Plant Molecular Genetics and Breeding / İzmir Institute of Technology, Turkey

Univ.-Prof. Dr. Wolfram Weckwerth Molecular Systems Biology / University of Vienna, Austria

 

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